Engineering of protein nanopores for sequencing, chemical or protein sensing and disease diagnosis
نویسندگان
چکیده
منابع مشابه
: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Insertional protein engineering for analytical molecular sensing
The quantitative detection of low analyte concentrations in complex samples is becoming an urgent need in biomedical, food and environmental fields. Biosensors, being hybrid devices composed by a biological receptor and a signal transducer, represent valuable alternatives to non biological analytical instruments because of the high specificity of the biomolecular recognition. The vast range of ...
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Current work in the Martin group has focused on utilizing single conical nanopores prepared by the track-etch method [1] to detect proteins via the resistive pulse sensing technique by recording current-time (i-t) traces through a single pore. When an analyte passes through the pore, translocation events are observed in the i-t trace. In this study, we recorded translocation events for an indiv...
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This chapter is focused on the development of experiments and theory of using solid-state nanopores for sensing single protein molecules in their native and unfolded states. Proteins serve diverse roles such as transport carriers, catalysts, molecular motors, cellular structural support, and others that make life possible. Because of these widely differing roles, proteins have an enormously div...
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ژورنال
عنوان ژورنال: Current Opinion in Biotechnology
سال: 2018
ISSN: 0958-1669
DOI: 10.1016/j.copbio.2017.11.006